Microscopic detection of phosphatase activity of saprophytic and arbuscular mycorrhizal fungi using a fluorogenic substrate
The location and activity of saprophytic and arbuscular mycorrhizal (NM) fungal phosphatases have been investigated with a fluorogenic substrate (ELF) that forms a fluorescent crystalline precipitate at the site of phosphatase activity. This ELF substrate method was compared with other detection methods such as fast blue RR salt staining and p-nitrophenyl phosphate. The ELF substrate was successfu