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In vitro evidence of root colonization suggests ecological versatility in the genus Mycena
The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic
The bacterial microbiota in first-void urine from men with and without idiopathic urethritis
BACKGROUND: Non-gonococcal urethritis (NGU) is a common syndrome in men. NGU may have several causes, but many cases are caused by sexually transmitted infections that may also cause complications in their female partners. Chlamydia trachomatis and Mycoplasma genitalium are the most common causes of NGU, but in up to 35% of the cases, none of the known viral or bacterial causes are found. Traditio
Towards diagnostic metagenomics of Campylobacter in fecal samples
BACKGROUND: The development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. Some of the challenges in diagnostic metagenomics are, that it requires a great next-generation s
Aminobacter MSH1-Mineralisation of BAM in Sand-Filters Depends on Biological Diversity
BAM (2,6-dichlorobenzamide) is a metabolite of the pesticide dichlobenil. Naturally occurring bacteria that can utilize BAM are rare. Often the compound cannot be degraded before it reaches the groundwater and therefore it poses a serious threat to drinking water supplies. The bacterial strain Aminobacter MSH1 is a BAM degrader and therefore a potential candidate to be amended to sand filters in w
Ultrastructure and phylogenetic position of Regin rotiferus and Otto terricolus genera et species novae (Bicosoecida, Heterokonta/Stramenopiles)
We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terri
Local diversity of heathland Cercozoa explored by in-depth sequencing
Cercozoa are abundant free-living soil protozoa and quantitatively important in soil food webs; yet, targeted high-throughput sequencing (HTS) has not yet been applied to this group. Here we describe the development of a targeted assay to explore Cercozoa using HTS, and we apply this assay to measure Cercozoan community response to drought in a Danish climate manipulation experiment (two sites exp
Groundwater chemistry determines the prokaryotic community structure of waterworks sand filters
Rapid sand filtration is essential at most waterworks that treat anaerobic groundwater. Often the filtration depends on microbiological processes, but the microbial communities of the filters are largely unknown. We determined the prokaryotic community structures of 11 waterworks receiving groundwater from different geological settings by 16S rRNA gene-based 454 pyrosequencing and explored their r
A three-gene phylogeny of the Mycena pura complex reveals 11 phylogenetic species and shows ITS to be unreliable for species identification
Phylogenetic analyses of Mycena sect. Calodontes using ITS previously suggested ten cryptic monophyletic ITS lineages within the Mycena pura morphospecies. Here, we compare ITS data (645 bp incl. gaps) from 46 different fruit bodies that represent the previously described ITS diversity with partial tEF-1-α (423 bp) and RNA polymerase II (RPB1) (492 bp) sequence data to test the genealogical concor
On the utilization of monitoring data in an ultimate limit state reliability analysis
This paper describes a structural reliability analysis utilizing monitoring data in the ultimate limit state with consideration of the uncertainties of the monitoring procedure. For this purpose the uncertainties of the monitoring data are modeled utilizing a new framework for the determination of measurement uncertainties. The approach is based on a process equation and statistical models of obse
Support structure reliability of offshore wind turbines utilizing an adaptive response surface method
This paper focuses on a reliability analysis of an offshore wind turbine support structure which is part of an assessment and monitoring framework for wind turbines in operation. The reliability analysis builds upon structural, loading, limit state and uncertainty models comprising design, production and erection data. This model basis facilitates the reliability analysis of the ultimate, the fati
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The present paper serves as an overview of recent developments by the author on the assessment and quantification of robustness of structures; the paper aims in this sense not to present any new results but collects approaches and results from previous publications. A general decision theoretical approach to the assessment and management of structural robustness is outlined. Structural robustness
Integrated monitoring of offshore wind turbines - requirements, concepts and experiences
Wind turbines on offshore sites (OWECs) are subjected to combined loads from wind and waves. These dynamic loads, with a frequency content within the range of the natural frequencies of the structures, cause fatigue-effective stresses in the substructures of wind turbines. Therefore, the examination of natural frequencies is an important part within the design process of wind turbines. The quality
Assessment and monitoring of reliability and robustness of offshore wind energy converters
The preseni paper provides an overview of existing approaches for design and assessment of offshore structures and on this basis proposes a framework for the risk based assessment and monitoring of the performance of offshore wind energy converters in operation. In recognition of the fact that there are presently no established best practices for the assessment and monitoring of offshore wind ener
Successional trophic complexity and biogeographical structure of eukaryotic communities in waterworks' rapid sand filters
As groundwater-fed waterworks clean their raw inlet water with sand filters, a variety of pro-and eukaryotic microbial communities develop on these filters. While several studies have targeted the prokaryotic sand filter communities, little is known about the eukaryotic communities, despite the obvious need for knowledge of microorganisms that get in contact with human drinking water. With a new g
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The structure of wind energy converters secures a reliable energy production. Hence an adequate reliability of the structure and additionally permanent assessment and maintenance is required. Within this paper methods for the assessment of the important damage mechanism fatigue in combination with monitoring of the fatigue reliability utilizing damage indicators are proposed. These methods compris
Amyloidity is not diagnostic for species in the Mycena pearsoniana complex (Mycena sectio Calodontes)
In Mycena sectio Calodontes with otherwise amyloid spores, the inamyloid spores of Mycena pearsoniana Dennis ex Singer were a distinguishing feature for this species and its subsection Violacella. Although the original concept of this species was European, Singer chose to typify it with material collected in Mexico. The name has since been applied to all European collections with inamyloid spores
Unilateral nuclear migration in Basidiomycetes : pheromone interaction, genomic conflicts and mating-system reversion
Unilateral nuclear migration (UNM) is a striking deviation from the normal reciprocal exchange of nuclei in mating between otherwise compatible monokaryons in Basidiomycetes. UNM has rarely been subject to systematical investigation. Here, we examine the scientific history of UNM in Basidiomycetes and evaluate the explanations brought forward. We suggest that UNM could have an evolutionary role in
A comparison between ITS phylogenetic relationships and morphological species recognition within Mycena sect. Calodontes in Northern Europe
The members of Mycena sect. Calodontes (Tricholomataceae s. l., Basidiomycota) are characterised by a collybioid aspect and more or less purplish to reddish colours and a distinct raphanoid odour. In Europe, nine species have been recognised though some of these based on somewhat dubious morphological differences. Historically, most were assigned to Mycena pura. However, since Mycena pura displays