In situ visualization of the effect of a pulsed electric field on plant tissue
A new method was developed for in situ visualization of changes related to electropermeabilization of plant tissue. Onion epidermis stained with neutral red was subjected to a pulsed electric field (PEF), and serial images were captured by a camera connected to a stercomicroscope. Sample resistance was recorded simultaneously. Above a threshold level of a field strength of 0.35 kV/cm, it was possi