The effects of phlorizin on several parameters of β-cell function were studied with microdissected islets of obese-hyperglycemic mice. At a concentration of 10 mM, phlorizin significantly depressed insulin release, glucose transport, glucose oxidation, and the level of fructose 1,6-diphosphate, when tested in the presence of 10 mM glucose. Whereas 1 mM phlorizin inhibited glucose transport by abou

An in vitro system with microdissected mouse islets was employed for studying insulin release. Islets from obese-hyperglycemic mice were considered particularly useful in view of their high content of adequately functioning β-cells. After freeze-drying and weighing each of the incubated islets it was possible to express the rate of insulin release per islet dry weight. Insulin released from a sing

The insulin-releasing ability and uptake characteristics of non-metabolizable, transport-specific amino acids were studied in an in vitro system, using microdissected pancreatic islets with more than 90% β-cells. Among the four stereoisomers of 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (BCH), only the b(-) form stimulated insulin release. This isomer is known as a specific substrate for trans

The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by m

Previous studies have revealed the existence of two separate phases in the glucose-stimulated insulin release. Exposure of microdissected mouse islets to mannoheptulose or epinephrine effectively inhibited both the initial transient release and the second persistent phase of insulin secretion. These results suggest that metabolism of glucose is a prerequisite for both phases of insulin release. Th

The pancreatic islets in obese-hyperglycemic mice display a normal response of insulin secretion when stimulated with glucose in vitro. This indicates that the impaired glucose metabolism is due to extra-pancreatic factors rather than to deficient β-cell function. In fact, the pancreas of these mice is a useful source from which to isolate large numbers of mammalian β-cells suitable for studies of

The release of insulin in vitro from isolated mouse islets was significantly inhibited in the presence of an islet protein extract equivalent to more than 100 times the normal serum level of insulin. The nature of the inhibitory islet substance remains unclear. The possibility that the blood circulation through the islets may be important for the local regulation of insulin release by reducing hig

Multidisciplinary collaboration between several European dermatology departments has identified isobornyl acrylate (IBOA; CAS 5888-33-5), once deemed a low-risk sensitizer, as a major culprit contact allergen in glucose sensors and insulin pumps, medical devices used by diabetes patients worldwide. Although the patch test modalities of IBOA have been fairly well characterized, intriguing questions

To enable realistic studies of massive multiple-input multiple-output systems, the COST 2100 channel model is extended based on measurements. First, the concept of a base station-side visibility region (BS-VR) is proposed to model the appearance and disappearance of clusters when using a physically-large array. We find that BS-VR lifetimes are exponentially distributed, and that the number of BS-V

Monte Carlo Event Generators are important tools for the understanding of physics at particle colliders like the LHC. In order to best predict a wide variety of observables, the optimization of parameters in the Event Generators based on precision data is crucial. However, the simultaneous optimization of many parameters is computationally challenging. We present an algorithm that allows to tune M

A wideband 65-nm CMOS power amplifier (PA) is presented, with a decade frequency range from 600 MHz to 6.0 GHz. In this frequency range, the output power exceeds 26.6 dBm and the power gain and power-added efficiency (PAE) exceed 18.1 dB and 49%, respectively. For a 7.5-dB peak-to-average-power-ratio (PAPR) long term evolution (LTE) signal at 1.9 GHz, the circuit provides an average output power o

Evaluation of other cultures is a strong force in a culture's definition of itself. Cultures are formed in encounters that include domination, conflict, and dismissal as much as appreciation and smooth exchange. In this paper, the construction of cultural identity is discussed, with reference to a Scandinavian Theme Park proposal made in cooperation between American design consultants and a local