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Foam generated by vigorous stirring of a nonionic detergent, Triton X-114, was used for the recovery of recombinant cutinase expressed by Saccharomyces cerevisiae. The enzyme with a hydrophobic fusion tag, (Trp-Pro)(4), was recovered with a higher yield as compared to the wild-type cutinase, indicating the involvement of hydrophobic interactions in protein isolation with the foam. The influence of
A general methodology for noise reduction and contrast enhancement in very noisy image data with low dynamic range is presented. Video footage recorded in very dim light is especially targeted. Smoothing kernels that automatically adapt to the local spatio-temporal intensity structure in the image sequences are constructed in order to preserve and enhance fine spatial detail and prevent motion blu
System verification and validation are important activitiesin the software development process aiming at testing thesoftware system in various quality aspects.The purpose of this study is to investigate theimportant characteristics of the verification andvalidation activities in the software development processin an organization. A qualitative research method isemployed in order to facilitate the
This paper analyzes the phase noise of the widely used differential LC oscillator topology and presents brief equations describing phase noise over the entire oscillation period. The findings show that if a capacitance is present at the source node of the differential pair, two phenomena occur that increase the phase noise: the switch time increases, lengthening the interval spent in a noisy balan
The study considers relations in a group of 95 managers, 41 women and 54 men, between managerial type and signs of anxiety and defense in the Defense Mechanism Technique modified (DMTm). Eight managerial types were distinguished, based on ratings of the managers by their subordinates in terms of the Ekvall and Arvonen (1991) leadership style dimensions of employee-centeredness, change-centeredness
We state limit distribution results for the isotonic inverse estimator of a distribution function when the data are disturbed by a random variable with a decreasing density. Three different dependence structures are considered: independent, weak dependent and suboordinated gaussian long range dependent data.
Objective: To perform detailed assessments of craniofacial dysmorphology in individuals with schizophrenia and controls in Sweden, in order to further elucidate the neurodevelopmental origins of schizophrenia. Method: We performed detailed, anthropometric assessments of craniofacial dysmorphology in male patients with schizophrenia (n = 24), healthy controls (n = 16), and patients' siblings with s
To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulf
A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the μM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-
Time-domain Green dyadics for linear, homogeneous, temporally dispersive bi-isotropic media are presented. A complex time-dependent electromagnetic field is introduced. Approximation to the complex field from an electric point dipole in an unbounded bi-isotropic medium with respect to the slowly varying components (second forerunner approximation) is obtained. Numerical examples are presented. Sur
The temperature dependencies of the intensities of the HCl stretching bands of the hydrogen chloride complexes with acetylene and ethylene have been used to obtain estimates of the dissociation enthalpies of the two complexes. Quantum chemical calculations on the Hartree Fock and DFT/B3LYP levels of theory have been combined with experimental data to give estimates of the intensities of the HCl st
The adsorption of whey proteins on modified and unmodified 316 2R stainless steel surfaces was studied by in situ ellipsometry under well-defined flow conditions. The effects of bulk properties, e.g. temperature, pH, protein concentration and flow rate as well as surface modification by ion implantation, sputtering, chemical vapour deposition and silica sol-gel deposition, were investigated. The s
Objectives. To record the occurrence of well-differentiated bladder carcinoma reported as T1 G1 to the Swedish Urinary Bladder Cancer Registry, to review the staging and grading of the carcinomas and to scrutinize the validity of the data on T1 G1 tumours reported to the registry during the period 1997-2000. Material and methods. The primary histopathological diagnoses of 98 cases of well-differen
