Serpentinite-hosted ecosystems are potential sites where life may first have evolved on Earth. Serpentinization reactions produce strongly reducing and highly alkaline fluids that are typified by high concentrations of molecular hydrogen (H2) and methane (CH4), which can be used as an energy source by chemosynthetic life. Low-temperature serpentinization at slow-spreading mid-ocean ridges provides

Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expression systems for heterologous protein production in many laboratories. The availability of robust insect cell lines, serum-free media, a range of vectors and commercially-packaged kits have supported the demand for maximizing the exploitation of the baculovirus-insect cell expression system. Naturally

Analyses of climate engineering (CE) governance have accelerated in the last decade. A key claim is that CE remains a largely ungoverned space, with shared norms, institutional arrangements, and formal rules to regulate CE not yet present. In contrast, here it is argued that de facto governance of CE is underway, discernible in an ordering of this nascent field of inquiry by unacknowledged sources

Repeat migration is a common, but unstudied, pattern of migration. This study examines the potential intergenerational consequences of this behavior. To investigate this, we estimate the effect of fathers’ repeat migration on their children’s grade point averages using population-level register data from Sweden. We find that the children of fathers who repeat migrate have a significantly lower gra

The remarkably efficient suppression of amyloid fibril formation by the DNAJB6 chaperone is dependent on a set of conserved S/T-residues and an oligomeric structure, features unusual among DNAJ chaperones. We explored the structure of DNAJB6 using a combination of structural methods. Lysine-specific crosslinking mass spectrometry provided distance constraints to select a homology model of the DNAJ

The brain is composed of hundreds of different neuronal subtypes, which largely retain their identity throughout the lifespan of the organism. The mechanisms governing this stability are not fully understood, partly due to the diversity and limited size of clinically relevant neuronal populations, which constitute a technical challenge for analysis. Here, using a strategy that allows for ChIP-seq

Liquid-liquid flow patterns and slug hydrodynamics were experimentally studied in non-circular glass microchannels with water-butanol, water-toluene, water–oil and water-hexane systems, considering various hydraulic diameters (600 µm, 400 µm, 200 µm), inlet junctions (crossed, T) and aspect ratios (0.5, 1). The aqueous phase was the continuous phase due to the hydrophilic microchannel walls and th

The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The phy

Landscape heterogeneity is an important driver of biodiversity in agroecosystems. However, the functional heterogeneity of agricultural landscapes, taking into account the different resources that habitat patches can provide to species, has rarely been studied. In this study, we explored the effect of landscape-scale nest availability provided by permanent grasslands on wild bee communities. Wild

För 300 år sedan fanns väldiga skogar med rik biologisk mångfald i Nordamerika. Tre pionjärer som dokumenterat denna idag till stora delar försvunna värld var Mark Catesby, Alexander Wilson och John James Audubon.

The γ-radiation by electrons with the energy of ∼200 MeV in a 100μm-thick diamond crystal has been measured at the MAX-lab experimental facility, when the electrons are incident on the crystal along the 〈100〉 axis. In this case, the intensity of the -radiation with the energy ∼1–2 MeV is approximately 16 times higher than that in amorphous matter of the same thickness. Theoretical calculations bas

This paper investigates the moisture dependency of ion diffusion in binary cementitious binders with Portland cement (OPC) in combination with slag or silica fume. Resistivity measurements and calculations with a thermodynamic model, GEMS, were used to assess the unsaturated chloride diffusion coefficient. The results show that the relation between the relative diffusion coefficient and the degree

The removal of natural organic matter (NOM) from boreal lake water by a novel capillary nanofiltration (NF) membrane was predicted using a computational fluid dynamics (CFD) modelling approach. The 2-dimensional axis-symmetric model was based on a 48 m3/day NF pilot plant operating in cross-flow mode on water containing 8 mg/L total organic carbon (TOC) at fluxes ranging from 10 to 25 L/m2/h and v

Background: Almost 1/3 of heart failure patients fail to respond to cardiac resynchronization therapy (CRT). A simple clinical score to predict who these patients are at the moment of referral or at time of implant may be of importance for early optimization of their management. Methods: Observational study. A risk score was derived from factors associated to CRT response. The derivation cohort wa

In an effort to strengthen midwifery students’ learning process, written reflections during the students’ clinical practice at birth units have been applied. The aim of this study was to examine how students of midwifery experienced the writing of daily reflections on their practice at birthing units. An interview study was carried out using an inductive method with descriptive design. During 2013

This work is a proof of concept of how a sequence of industrial batch separation steps together are used to form an integrated autonomous downstream process. The sequence in this case study consisted of an anion chromatography step, virus inactivation and finally a hydrophobic chromatography step. Moving from batch to integrated separation minimizes hold‐up times, storage tanks, and required equip

We present a microscopic model for collective effects in high multiplicity proton–proton collisions, where multiple partonic subcollisions give rise to a dense system of strings. From lattice calculations we know that QCD strings are transversely extended, and we argue that this should result in a transverse pressure and expansion, similar to the flow in a deconfined plasma. The model is implement