Förbättring av plasmidtransfektion till celler är av yttersta vikt vid forskning och behandling av sjukdomar baserat på genetiska tillvägagångssätt. Utvecklingen av omfattande, säkra, effektiva och skalbara metoder att införa genetiskt material i celler utgör en teknisk utmanande uppgift. Sonoporering, där trycket från ultraljudvågor orsakar perforering i cellmembranet, vilket möjliggör transfektiImproving plasmid transfection into cells is crucial in genetic disease research and treatment. The development of comprehensive, safe, effective, and scalable methods for introducing genetic material into cells poses a technically challenging task. Sonoporation, which utilizes pressure from ultrasonic waves to induce perforations in the cell membrane, enabling transfection, is an evolving method

Syftet med denna studie var att undersöka sambanden mellan attityder till miljön, personlighet och empati, samt huruvida personlighet och empati kan predicera miljöattityder. Detta gjordes genom en tvärsnittsstudie där målgruppen utgjorde unga vuxna i åldrarna 18-30 som rekryterades genom ett bekvämlighetsurval på sociala medier. I studien deltog 100 respondenter och svar samlades in genom ett fråThe purpose of this study was to investigate the relationships between environmental attitudes, personality and empathy, and whether personality and empathy can predict environmental attitudes. This was done through a cross-sectional study where the target group was young adults aged 18-30 who were recruited through convenience sampling on social media. 100 respondents participated in the study an

This study focuses on irregular migratory movements, and the mistreatment suffered by migrants, at the Southern Frontier of Europe, specifically at the Spanish-Moroccan border, from an international criminal law approach. The main question is whether the conduct of the security forces against migrants can be considered crimes against humanity and should therefore be investigated. The “problem” of

Bio-based and ecological buildings are gaining global importance, with straw bale constructions as one of the possible choices in Europe. Straw bale buildings are classified into load-bearing, non-load-bearing, and hybrid techniques. Load-bearing and non-load-bearing methods have been studied extensively in past decades whilst hybrid methods, such as CUT (Cells Under Tension) techniques have been

Historically, the correlation between stocks and bonds has exhibited stable patterns, often maintaining either a positive or negative sign over extended periods. Empirical evidence also shows, however, that this correlation tends to fluctuate due to various economic factors. This thesis aims to explore the presence of variation over time in the stock-bond correlation and to identify the factors re

Research question: How do consumer motivations differ in response to the use of the term vintage compared to second-hand, influencing willingness to buy? Purpose: The purpose of this thesis is to investigate young adults’ motivations and willingness to buy in the context of preowned fashion. Through a comparative study, from a consumer perspective, this study aims to understand how terms like seco

In current oat beverage production, insoluble components such as fiber fractions (plant cell structures) are usually decentered from the product, causing many types of loss. Retaining these fractions requires an efficient breakage of them, to avoid sandy mouthfeel in the end-product, which can be achieved for instance by homogenization. To study the homogenization efficiency, the resulted particle

Barnhandel är rekrytering, transport, överföring, inhysande eller mottagande av ett barn i syfte att exploatera hen. I Sverige, som i första hand är ett destinationsland för barnhandel, identifieras ett 50-tal barn per år som offer för människohandel. Antalet okända fall uppges dock vara betydligt högre. Med barn re-trafficking avses varje situation där ett barn som har brutit sig loss från en mänChild trafficking is the recruitment, transportation, transfer, harbouring, or receipt of a child for the purpose of exploitation. In Sweden, which primarily is a country of destination for child trafficking, around 50 children per year are identified as child victims of human trafficking. However, the number of unknown cases is said to be significantly higher. Child re-trafficking means any situa

Infärgning av vita blodkroppar för att möjliggöra blodanalys Kvantifiering av vita blodkroppar kan vara en snabb och enkel lösning på att diagnostisera olika sjukdomstillstånd. Genom att färga in de vita blodkropparna i ett blodprov kan man lättare analysera dem och då få kunskap om hur de ser ut och kunna räkna dem. Men vilken färg är faktiskt bäst för att färga in vita blodkroppar och vad finns​​The white blood cells are critical for the immune response of the body and the defence against infections. Measurements of the number of white blood cells in the blood is essential when diagnosing for example immunodeficiencies and determining the type of infection. Two types of measurements are frequently used, total white blood cell count and white blood cell differential. Both types are depen

För tidigt födda och sjuka barn hamnar på neonatal avdelning där de ofta behandlas med kombinationer av flera olika intravenösa läkemedel. Att kombinera intravenösa läkemedel innebär dock en risk för inkompatibilitet i form av fällningar och subvisuella partiklar, vilket kan riskera i livshotande tillstånd så som till exempel funktionssvikt i flera organ samt allvarliga hjärt- och kärlproblem. SoThere is a lack of data concerning compatibility between intravenously administered drugs, especially for doses administered in neonatal care. Co-administrating drugs or other substances used in hospital care, for instance parenteral nutrition that are incompatible with each other can, in worst case scenarios, lead to fatal outcomes. It is not only important to study drug incompatibility at differ

Syftet med uppsatsen är att undersöka möjligheter och utmaningar som mindre svenska kommuner har haft vid implementering av Robotic Process Automation (RPA). För att uppnå syftet med studien har tidigare litteratur kombinerats med en kvalitativ undersökning av tre kommuners erfarenheter kring RPA i kommunen. Resultatet av undersökningen visar att kommunerna har haft både utmaningar och möjligheter

Since the release of the recent generation of Large Language Models (LLMs) such as GPT-4 (GPT standing for generative pre-trained transformer), companies and individuals are exploring opportunities to improve their effectiveness by automating or receiving assistance with knowledge-intensive tasks by using artificial intelligence (AI). Within software engineering, a plethora of commercial services

This thesis explores the investment criteria applied by venture capitalists (VCs) for early-stage Deep Tech hardware startups in Europe, focusing on how these criteria and their characteristics vary across Pre-Seed, Seed, and Series A funding rounds. Drawing from a dataset of 102 relevant investment rounds spanning from 2022 to 2024, the study employs quantitative methods to analyse funding dynami

I dag finns transistorer över allt. De kan fylla olika funktioner och därför används också olika material och olika design. För att undersöka hur material och design påverkar prestandan är simuleringar ett viktigt verktyg, vilket denna mastersuppsats ämnar att undersöka. I en modern telefon finns flera miljarder transistorer. De är oftast några nanometer stora och gjorda i kisel. Dessa är anpassIn this thesis a simulation of a vertical GaN FinFET is created. The mobility models are first calibrated against known measurements of a real device. The model is then used to investigate the effects of scaling the device. It is shown that a 1 µm drift layer with a 1 µm fin could achieve a breakdown voltage of 300 V and for a 5 µm drift layer a breakdown voltage of 700 V can be reached. The resul

With an increasing emphasis on sustainability, and the necessity to align with new regulations, companies face the challenge of transforming their business models to incorporate circularity. Many companies today operate according to a linear business model, where products are designed for consummation and disposal. However, to comply with new demands surrounding sustainability, companies will need

An interacting particle system is a stochastic process describing the evolution of finitely or infinitely many particles, each of which, in the absence of any interaction, would evolve like a Markov process. The particle system can be examined in different scales by grouping particles together and considering their average. Their behaviour is described by a Markov process on a microscopic scale, w

Stålramar består av pelare och balkar och används i stora hallbyggnader som har stort avstånd mellan väggarna, till exempel lagerlokaler, eftersom stål är ett starkt material. När det kommer till att bygga stålramar används beräkningsprogram som ger förslag på hur stora pelarna och balkarna behöver vara för att klara av belastning från sin egen vikt, snö och vind. Det undersökta beräkningsprogrammWhen designing steel frames used in large hall buildings it is favourable to use a design tool set up with a programming language that can consider many different load cases and load combinations. These design tools can use the finite element method in order to perform the calculations needed to design the frames. Due to the extent of these design tools, the amount of elements used are restricted

There is a notable research gap in understanding the differential impacts of transnational versus domestic agricultural large-scale land acquisitions (LSLAs) on local biodiversity. This thesis addresses this gap within the context of Latin America and the Caribbean. Utilizing spatial panel datasets of the Biodiversity Habitat Index (BHI) from 2000 to 2020, LSLA area approximations, and a diverse s

It was a collision that appeared out of nowhere. Constructs representing different properties, forms, wills and histories appear in each other's view without leaving much time for reflection. More and more discussions about boundaries, meetings and connections are being held, more memories are being brought up and more fantasies about the future are being expressed on this land at the same tim

In recent decades, advancements in silicon technology have led to a substantial increase in chip complexity, primarily driven by the integration of more transis-tors within a given unit of chip area. Consequently, routing connections between these transistors has emerged as a significant challenge for the physical design of the chips. AMBA’s AXI-4 based connectivity is a commonly employed scheme t