The enzymatic activity of human carbonic anhydrase II (HCAII) was studied in the presence of nanoparticles of different nature and charge. Negatively charged nanoparticles inhibit HCAII whereas no effect is seen for positively charged particles. The kinetic effects were correlated with the strength of binding of the enzyme to the particle surface as measured by ITC and adsorption assays. Moreover,

The interaction between polyelectrolytes and counterions in confined situations and the mutual relationship between chain conformation and ion condensation is an important issue in several areas. In the biological field, it assumes particular relevance in the understanding of the packaging of nucleic acids, which is crucial in the design of gene delivery systems. In this work, a simple coarse-grai

In this study, we investigate for the first time the efficiency of an environmentally sustainable extraction technique (pressurized liquid extraction, PIE) in conjunction with a fast separation technique (ultra-high performance liquid chromatography, UHPLC) coupled to a selective mass spectrometry (MS) detector (quadrupole time-of-flight, qTOF) to extract, separate and quantify fifteen intact-gluc

Although plesiosaurs and mosasaurs co-existed for about 35 million years at the end of the Cretaceous, the fossil record documenting interactions between these two groups of marine reptiles is meagre. The discovery of deeply incised scars on a limb bone of an immature polycotylid plesiosaur from the latest early Campanian (in the European two-fold division of the Campanian Stage) of the Kristianst

In this paper, standard-cell based memories (SCMs)are proposed as an alternative to full-custom sub-VT SRAM macros for ultra-low-power systems requiring small memory blocks. The energy per memory access as well as the maximum achievable throughput in the sub-VT domain of various SCM architectures are evaluated by means of a gate-level sub-VT characterization model, building on data extracted from

HC-128 is an eSTREAM final portfolio stream cipher. Several authors have investigated its security and, in particular, distinguishing attacks have been considered. Still, no one has been able to provide a distinguisher stronger than the one presented by Wu in the original HC-128 paper. In this paper we first argue that the keystream requirement in Wu’s original attack is underestimated by a factor

Obesity is a global problem and in aspects of lethal ethanol intoxications virtually unexplored. The cause of death in ethanol intoxication is generally considered to be suppression of the respiratory function. Previous research indicates that respiratory function is more vulnerable in obese subjects than in those of normal weight. We hypothesized that lethal blood alcohol concentration (BAC) is l

Differential diagnosis of parkinsonian disorders is challenging because of overlapping symptoms, especially during early stages of disease. No validated biomarkers are available for early and accurate diagnosis of multiple system atrophy and other parkinsonian disorders. It has been reported that flt3 ligand levels in cerebrospinal fluid could clearly differentiate patients with Parkinson's diseas

Interindividual variations of immunoglobulin constant heavy G chain (IGHG) genes on chromosome 14q32.3 are identified by alternative genetic markers (GM) of IgG3, IgG1 and IgG2, respectively. They express structurally and functionally innate IgG molecules and B cells, associated with allergic disease, replicated in several studies.

Financial markets are complex processes where investors interact to set prices. We present a framework for option valuation under imperfect information, taking risk neutral parameter uncertainty into account. The framework is a direct generalization of the existing valuation methodology. Many investors base their decisions on mathematical models that have been calibrated to market prices. We argue

In development, epigenetic mechanisms such as DNA methylation have been suggested to provide a cellular memory to maintain multipotency but also stabilize cell fate decisions and direct lineage restriction. In this study, we set out to characterize changes in DNA methylation and gene expression during granulopoiesis using 4 distinct cell populations ranging from the oligopotent common myeloid prog

At northern latitudes, vitamin D is not synthesized endogenously during winter, causing low plasma 25-hydroxyvitamin D (25(OH)D) concentrations. Therefore, we evaluated the effects of a healthy Nordic diet based on Nordic nutrition recommendations (NNR) on plasma 25(OH)D and explored its dietary predictors. In a Nordic multi-centre trial, subjects (n = 213) with metabolic syndrome were randomized

PURPOSE: This cross-national qualitative study explores how very old people reflect upon relocation and aging in place. DESIGN AND METHODS: Swedish and German data are utilized in this study. About 80 community-living participants, 80-89 years of age, were interviewed with open-ended questions at home by trained interviewers. The interviews were analyzed by using conventional qualitative content a

Malignant cells, like all actively growing cells, must maintain their telomeres, but genetic mechanisms responsible for telomere maintenance in tumors have only recently been discovered. In particular, mutations of the telomere binding proteins alpha thalassemia/mental retardation syndrome X-linked (ATRX) or death-domain associated protein (DAXX) have been shown to underlie a telomere maintenance

Nebel D, Bratthall G, Ekblad E, Norderyd O, Nilsson B-O. Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01382.x. © 2011 John Wiley & Sons A/S Background and Objective: Estrogen acts via estrogen receptor (ER) α and β. The expression pattern of ERs and their importanc

The ability to anticipate future events and to modify erroneous anticipatory actions is crucial for the survival of any organism. Both theoretical and empirical lines of evidence implicate the cerebellum in this ability. It is often suggested that the cerebellum acquires "expectations" or "internal models." However, except in a metaphorical sense, the cerebellum, which consists of a set of interco

Plumage constitutes a significant component of the somatic investment of birds. A detailed investigation of feathers and moult can help to uncover trade-offs involved in somatic investment decisions, the sources of some of the costs birds have to pay and the potential fitness consequences. We used micro-computed tomography imaging to study the second moment of area, a structural parameter that is

A low-background inclusive search for new physics in events with same-sign dileptons is presented. The search uses proton-proton collisions corresponding to 20.3 fb(-1) of integrated luminosity taken in 2012 at a centre-of-mass energy of 8TeV with the ATLAS detector at the LHC. Pairs of isolated leptons with the same electric charge and large transverse momenta of the type e(+/-) e(+/-), e(+/-) mu

This article explores the different ways in which the nuclear catastrophe at the Chernobyl plant in 1986 is represented in selected films and novels produced and written since the fall of the Soviet Union in 1991. The most common way of imagining Chernobyl during this period is the apocalypse foreshadowing the collapse of the Soviet Union. However, it is also viewed as the catalyst for resurrectio