Model Calibration and Optimization of a Protein Purification Process
Four different chromatography models were calibrated to describe the separation of a ternary protein mixture consisting of lysozyme, cytochrome C and ribonuclease A in an ion-exchange chromatography column. The models are based on the same column model, the kinetic dispersive model. Protein adsorption was described by four different adsorption models, the Langmuir model with mobile phase modulator