Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic st

Septic lung damage is associated with endothelial cell and neutrophil activation. This study examines the role of the E3 ubiquitin ligase midline 1 (Mid1) in abdominal sepsis. Mid1 expression was increased in endothelial cells derived from post-capillary venules in septic mice and TNF-α challenge increased Mid1 levels in endothelial cells in vitro. The siRNA-mediated knockdown of Mid1 decreased TN

Sexual harassment (SH) and other forms of mistreatment continue to be a significant problem at workplaces, leading to negative health and work-related outcomes. Previous studies have mainly examined SH and other types of workplace harassment separately. In this study we investigated whether harassment related to any of the seven Swedish legal grounds for discrimination (sex, transgender identity o

Mixed cropping systems can constitute important agroecological adaptation strategies for enhancing crop growth and productivity in view of climate change, while reducing the need for synthetic fertilizers and providing important ecosystem services. The aim of this study was to investigate growth, competitiveness, and productivity of two forage mixtures combining triticale (X triticosecale Wittmack

Objective: To determine the risk of comorbidity following diagnosis of knee or hip osteoarthritis (OA). Design: A cohort study was conducted using the Integrated Primary Care Information database, containing electronic health records of 2.5 million patients from the Netherlands. Adults at risk for OA were included. Diagnosis of knee or hip OA (=exposure) and 58 long-term comorbidities (=outcome) w

Objectives: Efficacy and safety results from the EMERGE (NCT02484547) and ENGAGE (NCT02477800) phase 3 studies of aducanumab in early Alzheimer’s disease (AD) have been published. In EMERGE, but not in ENGAGE, high-dose aducanumab demonstrated significant treatment effects across primary and secondary endpoints. Low-dose aducanumab results were consistent across studies with non-significant differ

Chemical interactions that drive crater wear in turning are often studied using diffusion couples where the tool and workpiece are fixed. In contrast, in actual turning, there is a constant supply of new workpiece material at the tool-chip interface. In this work, diffusion simulations of a WC–Co(6%) and Ti–5Al–5V system were conducted, with constant replenishment of titanium at the interface (ope

The purpose of this study is to provide a system perspective on the barriers to the development of a fossil-free and thus more sustainable urban freight system and to provide insights regarding these barriers and how they impede progress. The system perspective complements earlier research, where barriers were identified in delimited parts of freight systems. Here, the urban freight system in a Sw

Cyanobacterial blooms are becoming more frequent in freshwater sources, causing concern throughout the world. Cyanobacterial blooms affect human health and the entire environment. Numerical modeling is an effective tool for investigating aquatic systems. In this study, a 3D hydrodynamic and water quality (ecological) model was used to simulate eutrophication of a drinking water source, Lake Vomb,

Knowledge of how biodiversity sustains ecosystem function comes predominantly from studies focused on small spatial scales. Thus, we know relatively little about the role of biodiversity at larger scales of space and time where habitats become increasingly heterogeneous. Efforts to upscale the relationship between biodiversity and function have yielded inconclusive results. Given that increasing h

This paper presents an LO phase shifter with frequency tripling for 28-GHz 5G transceivers. The phase shifting and frequency tripling are achieved using an injection-locked oscillator and injection-locked frequency tripler, respectively. A phase detector based on third harmonic mixing is also implemented and is used to detect the applied phase shift, supporting automatic calibration of the phase s

Epithelioid and spindle cell hemangioma was initially described in 1999 in a series of primary bone tumors and was subsequently suggested by some to represent a variant of epithelioid hemangioma. Here, we studied 18 epithelioid and spindle cell hemangiomas. Nine patients (50%) were male. Age at presentation ranged from 12 to 78 years (median: 38.5 y). Nine patients (50%) had tumor(s) limited to bo

Imidacloprid is a neonicotinoid neurotoxin that remains widely used worldwide and persists in the environment, resulting in chronic exposure to non-target insects. To accurately map dose-dependent effects of such exposure across taxa, toxicological assays need to assess relevant modes of exposure across indicator species. However, due to the difficulty of these experiments, contact bioassays are m

Three-dimensional city models are increasingly being used for analyses and simulations. To enable such applications, it is necessary to standardise semantically richer city models and, in some cases, to connect the models with external data sources. In this study, we describe the development of a new Swedish specification for 3D city models, denoted as 3CIM, which is a joint effort between the thr

Insulin resistance causes compensatory insulin production, which in humans can eventually progress to β-cell failure and type 2 diabetes (T2D). This disease progression involves multi-scale processes, ranging from intracellular signaling to organ and whole-body level regulations, on timescales from minutes to years. T2D progression is commonly studied using overfed and genetically modified rodents

Background: Fatigue is a debilitating symptom of multiple sclerosis (MS), but its relation to sociodemographic and disease-related characteristics has not been investigated in larger studies. The objectives of this study were to evaluate predictors of self-reported fatigue in a Swedish nationwide register-based MS cohort. Methods: Using a repeated cross-sectional design, we included 2,165 persons

Sommaren 1767 påbörjade orientalisten Jacob Jonas Björnståhl (1731–1779) en resa som förde honom till Frankrike, Schweiz, Italien, Tyskland, Holland och England innan han sjövägen tog sig till Konstantinopel och så småningom Grekland, där han avled i dysenteri vid 48 års ålder. Hade det inte varit för tidningsmannen Carl Christoffer Gjörwell hade eftervärlden aldrig fått någon större kännedom om d

Co-design processes are of key importance in planning and implementing Nature-based Solutions (NBS), but require boundary management between diverse holders of local, practical and scientific knowledge. Participatory and mapbased planning tools, such as Geodesign workshops, can facilitate such boundary management through mobilising knowledge holders, and translating and negotiating between differe