The emerging field of proteomics has created a need for new high-throughput methodologies for the analysis of gene products. An attractive approach is to develop systems that allow for clonal selection of interacting protein pairs from large molecular libraries. In this study, we have characterized a novel approach for identification and selection of protein-protein interactions, denoted SPIRE (se

Escherichia coli is a microorganism routinely used in the production of heterologous proteins. The overexpression of a xylanase (Xyn10A Delta NC), which originated from the thermophile Rhodo-thermus marinus cloned under the control of the strong T7/lac promoter in a defined medium (mAT) using a substrate-limited feed strategy, was however shown to impose a significant metabolic burden on host cell

Abstract in UndeterminedThe nuisance alga Gonyostomum semen (Raphidophyceae) is known to form dense blooms in many freshwater bodies, and has increased its distribution and abundance. However, information on the mechanisms behind bloom formation and maintenance is scarce. Field observations indicate that G. semen may be favored in humic lakes, In the present study, we performed controlled laborato

Multireference complete active space self-consistent-field (CASSCF) and multireference CASSF second-order perturbation theory (MSCASPT2) calculations were performed on the ground state and a number of low-lying excited singlet and triplet states of chlorobenzene. The dual phosphorescence observed experimentally is clearly explained by the MSCASPT2 potential-energy curves. Experimental findings reg

The efficiency of different preparations of lipases was evaluated in organic solvents. Lipases from Humicola lanuginosa, Candida rugosa, Rhizomucor miehei and Pseudomonas cepacia were adsorbed onto the surfactant sorbitan monostearate (Span 60) and the specific activities were compared in hexane to crude powder (used straight from the bottle) and lipase freeze-dried from buffer solution. Lipases a

Genetic engineering has been used for the fusion of peptides, with different length and composition, on a protein to study the effect on partitioning in aqueous two-phase systems containing thermoseparating polymers. Peptides containing 2-6 tryptophan residues or tryptophan plus 1-3 lysine or aspartate residues, were fused near the C-terminus of the recombinant protein ZZT0, where Z is a synthetic

Pre-treatment serum levels of sCD163 were measured in a cohort of 236 suspected tuberculosis (TB) cases from Guinea-Bissau, with a median follow-up period of 3.3 years (range 0-6.4 years). In 113 cases, the diagnosis of TB was verified by positive sputum microscopy and/or culture. Among the verified TB cases, a decreased survival rate was found in 27 patients with sCD163 levels above the upper ref

Coherent structures and mixing in the flow field of a jet in crossflow have been studied using computational (large eddy simulation) and experimental (particle image velocimetry and laser-induced fluorescence) techniques. The mean scalar fields and turbulence statistics as determined by both are compared for circular, elliptic, and square nozzles. For the latter configurations, effects of orientat

Objective: The course and the long-term outcome of 'refractory' secretory otitis media (SOM), defined as continuous SOM for more than 6 years, were studied in 52 young patients. They had during childhood been treated for bilateral SOM on average for 12 years (range 6 to 26 years). The mean interval between resolution of SOM and examination was 7 years. Methods: At follow up the patients' medical r

Alcohol abuse is associated with an increased risk of osteopenia and fractures. Previous histomorphometric studies on iliac crest bone have found decreased bone formation and increased bone resorption in alcohol abusers but it has not been established whether alcohol abuse has any effect on the anatomical location or the healing time of tibial shaft fractures. We studied, retrospectively, 199 adul

Aim. The purpose of this investigation was to analyze predictors for wound healing, amputation and mortality after vacuum assisted closure (VAC (R)) therapy of wounds in the lower limb in patients with arterial disease. Methods. One hundred and twenty one wounds were treated and followed for 12 months at two vascular centres in Uppsala and Malmo, Sweden. VAC (R) therapy was applied in the wound at

Recent work in the literature had evaluated the energy-momentum tensor of a Casimir apparatus in a weak gravitational field, for an electromagnetic field subject to perfect conductor boundary conditions on parallel plates. The Casimir apparatus was then predicted to experience a tiny push in the upward direction, and the regularized energy-momentum tensor was evaluated to first order in the gravit

A method based on U-V-irradiation in organic solvent followed by transfer of the remaining pollutants into silicone oil for subsequent biodegradation in a biphasic system inoculated with a phenanthrene degrading Pseudomonas sp. was tested for the treatment of various mixtures of PAHs. Acetone was first selected as the most suitable solvent compared to methanol, acetonitrile and silicone oil for th

BACKGROUND: Cobalamin/folate deficiency is common in elderly subjects and may lead to psychiatric symptoms, but even more often it increases the severity of other organic and non-organic mental diseases. It is therefore of importance to evaluate the optimal use of different markers of cobalamin/folate status in a psychogeriatric population. METHODS: We measured serum cobalamin, blood folate, plasm

The large eyes and well-developed visual system of billfishes suggest that vision is an important sense for the detection and interception of prey and lures. Investigations of visual abilities in these large pelagic fishes are difficult, however anatomical studies of billfish eyes and retinas allow prediction of a number of visual capabilities. From the density of ganglion cells in the blue marlin

As part of the project of postcolonial national modernization, the United Arab Emirates has seen the transformation of significant areas of the country’s desert environment into green landscapes, with enormous resources devoted to agricultural development, park landscaping and nature reserves. In addition, recent years have also seen the creation of a number of social institutions dealing with env

During the summer of 1935, approximately 20,000 Swedish women mobilized in a peaceful action the Women's Unarmed Uprising Against War. The manifestation was a protest against rearmament and, in particular, against a militarization of everyday life that might result from a civil defense buildup. Thus, in a spirit of solidarity women were encouraged to refuse to use gas masks or evacuate into cellar