Recombinant Expression in Pichia pastoris and Characterization of GH36 α-Galactosidases
α-Galactosidases from Aspergillus nidulans (AglC) and Penicillium subrubescens (PsGal36-2527) are exo-acting enzymes that can be found in the Glycoside Hydrolase (GH) 36 phylogenetic cluster with eukaryotic α-galactosidases. In utilizing enzymes for industrial applications, finding enzymes with a broad range of substrate specificities and high catalytic efficiencies is quite challenging. It has be