The ability of lentiviral vectors to transfer genes into human hematopoietic stem cells was studied, using a human immunodeficiency virus 1 (HIV-1)-derived vector expressing the green fluorescence protein (GFP) downstream of the phosphoglycerate kinase (PGK) promoter and pseudotyped with the G protein of vesicular stomatitis virus (VSV). High-efficiency transduction of human cord blood CD34(+) cel

Human immunodeficiency virus type 1-based lentivirus vectors containing the green fluorescent protein (GFP) gene were used to transduce murine Lin(-) c-kit(+) Sca1(+) primitive hematopoietic progenitor cells. Following transduction, the cells were plated into hematopoietic progenitor cell assays in methylcellulose and the colonies were scored for GFP positivity. After incubation for 20 h, lentivir

OBJECTIVES: Lentiviral vectors can transduce nondividing cells. As most haematopoietic stem cells (HSCs) are nondividing in vivo, lentiviral vectors are promising viral vectors to transfer genes into HSCs.DESIGN AND SETTING: We have used HIV-1 based lentiviral vectors containing the green fluorescent protein (GFP) gene to transduce umbilical cord blood CD34+ and CD34+/CD38- cells prior to transpla

Lentiviral vectors are promising tools for the development of gene therapy since they can transduce both quiescent and dividing target cells. Lentiviral vectors may be particularly promising gene delivery tools for hematopoietic stem cells since these target cells tend to be quiescent and are therefore difficult target cells for vectors that require dividing targets. Human hematopoietic stem cells

Spin-cast intermolecular interactions in conjugated polymer films lead to the formation of excited states delocalized over a few oriented and tightly packed conjugated segments. The optoelectronic properties of conjugated polymers are strongly dependent on the presence of such oriented domains at a nanoscale level. We observe oriented domains as large as several micrometers in size spontaneously f

A survey experiment, carried out in a field setting among Sunni Arab Syrian refugees (N = 2,479), examined the effect of exposure to wartime trauma, ethnoreligious group affiliation, and degree of hostility of intergroup relations on altruism and positive emotional regard. The results showed that in-group targets were met with more positive emotional regard and altruism than relatively neutral out

Objectives. To evaluate the distribution and impact of ABO blood groups on postoperative outcomes in patients undergoing surgery for acute type A aortic dissection (ATAAD). Design. A total of 1144 surgical ATAAD patients from eight Nordic centres constituting the Nordic consortium for acute type A aortic dissection (NORCAAD) were analysed. Blood group O patients were compared to non-O subjects. Th

This chapter contains an outline of the Swedish social security system with a focus on sickness benefits in the social security and unemployment insurance. The Swedish legislator’s ambition has been to create parity between different forms of employment as well as between those who are self-employed and those who operate in different company structures. Legislative amendments in this area have mai

The precarious employment types growing most rapidly in Sweden are on-call with zero hour contracts, umbrella organizations and crowdwork in the gig economy. In the Swedish binary system, those in precarious work often fall between the regulations for employees and those for the self-employed. Both the legislator and the social partners in Sweden work with these problems in the field of employee p

Industrial relations in Sweden are part of the Nordic legal tradition. This is characterised by labour law which is largely semi-discretionary law and collective agreements are the most important instrument for regulation of the labour market; these agreements offer a high degree of coverage. Trade unions are well organised and play a central role in the labour law system. During the past 25 years

An in-depth understanding of the reaction mechanism is required for the further development of Mo-based catalysts for biobased feedstocks. However, fundamental studies of industrial catalysts are challenging, and simplified systems are often used without direct comparison to their industrial counterparts. Here, we report on size-selected bimetallic NiMo nanoparticles as a candidate for a model cat

Critical thinking is an important aim of higher education, and it is a skill particularly important in interdisciplinary fields. I am proposing a seminar where, based on an initial text and some central discussion questions, we can discuss our experiences with teaching and learning in an interdisciplinary manner. The experience from sustainability-related teaching shows that addressing interdiscip

Objectives: NEAT001/ANRS143 demonstrated non-inferiority of once-daily darunavir/ritonavir (800/100 mg) + twice-daily raltegravir (400 mg) versus darunavir/ritonavir + tenofovir disoproxil fumarate/emtricitabine (245/200 mg once daily) in treatment-naive patients. We investigated the population pharmacokinetics of darunavir, ritonavir, tenofovir and emtricitabine and relationships with demographic

My chapter gives an overview of gender and religion in Europe in the “long” 19th century based on developments in historical research since the 1980s. The focus is on Catholicism and Protestantism with some reflexions also on Judaism and Orthodox Christianity.

Det finns en stark traditionalistisk rörelse inom katolska kyrkan. Bakgrunden är de reformer som genomfördes som ett resultat andra vatikankonciliet 1962-1965. Konciliet banade väg för en mer öppen form av katolicism, som av många konservativt sinnade katoliker uppfattades som ett avsteg från den katolska tron och traditionen. För att värna vad man uppfattar som den sanna katolicismen har dessa tr

The focus of this chapter is the relationship between owners and hybrid organizations with focus on public-private hybrid constellation and corporations owned by local governments. These companies are hybrids since they are governed as private profit-oriented companies but provide public services and incorporate public values. The main takeaway from the analysis in the chapter is that two dimensio