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In mice, along with the assessment of eosinophils, lung function measurements, most commonly carried out by plethysmography, are essential to monitor the course of allergic airway inflammation, to examine therapy efficacy and to correlate animal with patient data. To date, plethysmography techniques either use intubation and/or restraining of the mice and are thus invasive, or are limited in their
When attempting to establish accurate models for the human diploe, micro-scale morphological differences in the four main areas of the calvaria could also be considered. In this study, X-ray computed microtomography (μ-CT) images were analyzed in order to quantitatively characterize the micro-architecture of the human calvarium diploe. A bone specimen from each area of the skull (temporal, frontal
Lung imaging in mouse disease models is crucial for the assessment of the severity of airway disease but remains challenging due to the small size and the high porosity of the organ. Synchrotron inline free-propagation phase-contrast computed tomography (CT) with its intrinsic high soft-tissue contrast provides the necessary sensitivity and spatial resolution to analyse the mouse lung structure in
Propagation-based X-ray phase-contrast computed tomography (PBI) has already proven its potential in a great variety of soft-tissue-related applications including lung imaging. However, the strong edge enhancement, caused by the phase effects, often hampers image segmentation and therefore the quantitative analysis of data sets. Here, the benefits of applying single-distance phase retrieval prior
Background/Objectives: There is still no consensus as to whether patients with upfront resectable synchronous colorectal liver metastases (sCRLM) should receive neoadjuvant treatment prior to liver surgery. Two randomized controlled trials have assessed the role of peri-operative chemotherapy in sCRLM; neither have shown a survival benefit in the neoadjuvant group. The aim of this population-based
Background: Klebsiella pneumoniae infections pose a great burden worldwide, causing high morbidity and mortality, which are worsened by the increase in multidrug-resistant strains. New therapeutic/prophylactic strategies are urgently needed to overcome antibiotic resistance and reduce the health and economic impacts of diseases caused by this pathogen. Fimbriae are important virulence factors invo
This study investigated the impact of a national palliative care policy introduced in 2013. The hypothesis was that end‑of‑life and palliative care policy shape healthcare services, which in turn influence service utilisation and ultimately place of death for people dying from cancer. The aim was to identify longitudinal trends in place of death for people with cancer in Sweden. A population‑level
Accurate models for the human diploe have to take into account any structural differences in the four main areas of the calvaria bones. In this study the technique of Xray computed microtomography (μ-CT) along with image analysis was used in order to visualize and quantitatively analyze differences in the micro-architecture of the human calvarium diploe. A bone specimen from each area of the skull
The non-coding genome, constituting 98% of human DNA, remains largely unexplored, yet holds potential for identifying new biomarkers and therapeutic targets in acute lymphoblastic leukemia (ALL). In this study, we conducted a systematic analysis of recurrent somatic non-coding single nucleotide variants (SNVs) in pediatric B-cell precursor (BCP) ALL. We leveraged whole genome sequencing (WGS) data
Plesiosaurs are charismatic marine reptiles of the Mesozoic Era. Life-like reconstructions of long-necked forms are typically ‘shrink-wrapped’ with a narrow, snake-like neck and a sea turtle-like body; a depiction that has changed little since the initial discovery of plesiosaurs over 200 years ago. Despite their long geological history and importance in founding paleontology as a scientific disci
This paper introduces heat semigroups of topological Markov chains and Cuntz–Krieger algebras by means of spectral noncommutative geometry. Using recent advances on the logarithmic Dirichlet Laplacian on Ahlfors regular metric-measure spaces, we construct spectral triples on Cuntz–Krieger algebras from singular integral operators. These spectral triples exhaust odd K-homology and for Cuntz algebra
We have used ≈ 40 fs pulses from a Ti:Sa laser to investigate the ultrafast energy transfer and relaxation dynamics in the LH1 and LH2 light-harvesting pigment-proteins of photosynthetic purple bacteria. Our results reveal a new feature, not earlier observed in light-harvesting pigments, ultrafast relaxation characterized by a time constant of approximately 20 fs, which we interpret as interexcito
Picosecond spectrally resolved fluorescent measurements together with the coupled kinetic rate equation model simulations of the data have been employed to determine the rates and pathways of heterogeneous excitation transport in the membranes of photosynthetic bacteria Rhodobacter sphaeroides and Chromatium minutissimum. Excitation transport from bacteriochlorophyll molecules B800 to B850, belong
A spectroscopic characterization of the chlorophyll a (Chl) molecule in the monomeric cytochrome b6f complex (Cytb6f) isolated from the cyanobacterium Synechocystis PCC6803 is presented. The fluorescence lifetime and quantum yield have been determined, and it is shown that Chl in Cytb6f has an excited-state lifetime that is 20 times smaller than that of Chl in methanol. This shortening of the Chl
The temperature dependence of absorption and selectively excited fluorescence spectra of B820 subunit of the bacterial light-harvesting antenna is modelled by using the impurity center theory ofoptical spectra. In order toget acceptable agreement between theory and experiment, we suggest that due to the dimeric nature of B820, the electronic transition is weakly coupled to a rather broad distribut
The picosecond time-domain singlet excitation transfer and trapping kinetics in photosynthetic membranes in case of low excitation intensities is studied by numerical integration of the appropriate master equation. The essential features of our two-dimensional-lattice random walk model are spectral heterogeneity of the light-harvesting antenna, inclusion of temperature effects, nonabsolute excitat
Polarized, site-selected fluorescence was measured for light-harvesting complex II (LHCII), the major Chl a/b/xanthophyll binding light-harvesting complex of green plants. Upon selective excitation in the range of 679-682 nm at 4 K, separate zero-phonon lines and phonon wings could be observed, as well as sharp lines in the vibronic region of the emission: vibronic zero-phonon lines. The maximum o
We investigated the exciton transfer dynamics in photosynthetic light-harvesting complex 2 (LH2) coupled to an optical microcavity. Using computational simulations based on Redfield theory, we analyzed how microcavity coupling influences energy relaxation and transfer within and between LH2 aggregates. Our results show that the exciton transfer rate between B850 rings follows a square dependence o
Absorption bands of photosynthetic and other biological pigment-protein complexes undergo dramatic variations in position and shape upon changing the temperature. In this paper we have reviewed a calculation algorithm to simulate the temperature dependence of electronic spectra coupled to any number of vibrations with arbitrary coupling strength. A number of computer simulations is carried out in
