Flow-ELISA measurements of the monoclonal antibody concentration in cultivation broth containing murine hybriboma cells were carried out using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A new specialised pellicular agarose/stainless steel matrix designed for high flow rates with fast mass transport properties was used. Special care was taken to get an efficient flow distr

A new type of agarose material, superporous agarose, was used as a support material in an analytical system designed for monitoring of bioprocesses with respect to metabolites and intracellular enzymes. The superporous agarose was used in the form of miniaturised gel plug columns (15×5.0 mM I.D. monolithic gel bed). The gel plugs were designed to have one set of very large pores (about 50 μm i

A superporous agarose matrix was compared with a corresponding homogenous matrix in the isolation of recombinant factor VIII SQ (r-VIII SQ) by immunoaffinity chromatography. As a reference, the commercially available Sepharose FastFlow, used for a similar purification in the industry, was also evaluated. Breakthrough curves are described for flows between 50 and 400 cm/h with pre-purified r-VIII S

A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the ant

Continuous agarose beds (monoliths) were prepared by casting agarose emulsions designed to generate superporous agarose. The gel structures obtained were transected by superpores (diameters could be varied in the range 20–200 μm) through which liquids could be pumped. The pore structure and the basic properties of the continuous gel were investigated by microscopy and size exclusion chromatogr

The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control

Flowing water impacts bridges over rivers primarily in two ways: (1) hydrodynamic forces that directly impact the structure and (2) scouring at piers and abutments, including contraction scour. The design prin-ciples for bridges in Sweden, including both impacting forces and local scour, are relatively simplistic. The purpose of this paper is to examine the impact of extreme river flows on bridges

The well-known green color of plants is due to the chlorophyll molecules used for light harvestingduring photosynthesis, which takes place in chloroplasts. Biosynthesis of chlorophyll a is performed by15 enzymatic steps and chlorophyll a can be interconverted to chlorophyll b via the chlorophyll cycle.Barley is a valuable model organism for studying chloroplast development and chlorophyll biosynth

Idag transporteras stora mängder gods runt om i världen med lastbilar och fartyg, vilket förväntas fortsätta även i framtiden då det är avgörande för samhällets funktion. Det vanligaste framdrivningssystemet för dessa fordon är kompressionständningsmotorn, även känd som dieselmotorn, som då vanligtvis drivs av fossilt dieselbränsle. Detta resulterar i betydande utsläpp som måste minskas, för att uIn today's world, the transportation of goods via trucks and ships plays a pivotal role, and this trend is expected to persist in the foreseeable future, owing to its critical importance to society. However, the most common propulsion system for these vehicles, the compression ignition (CI) engine, commonly known as the diesel engine, relies heavily on fossil diesel fuel, resulting in significant

This paper analyses the conditions and factors influencing non-university researchers' career transitions back into academia. Using affiliation data in publications of German researchers, their cross-sector career transitions from the public research organisation Fraunhofer-Gesellschaft and industry to universities are analysed. Although the rate of transitions is small, there are distinctive wind

Nos últimos anos, houve uma repressão contra defensores do meio ambiente em Uganda. Jornalistas, estudantes, agricultores e moradores da zona rural defendem o ambiente natural, protestando e mobilizando-se contra o tratamento injusto e a migração forçada causada por infraestruturas de combustíveis fósseis, agronegócio, energia hidrelétrica ou compensação de carbono. Essa situação colocou as autori

Purpose: Currently, foresight studies have been adapted to incorporate new techniques based on big data and machine learning (BDML), which has led to new approaches and conceptual changes regarding uncertainty and how to prospect future. The purpose of this study is to explore the effects of BDML on foresight practice and on conceptual changes in uncertainty.Design/methodology/approach: The method

As humanity faces multiple mutually-reinforcing social-ecological crises, rhetoric on transformations abounds. However, transformations are normative, politically-contested processes that risk perpetuating, or even exacerbating, the ecological and socio-economic crises of today. We argue that transformations should seek to enhance human and non-human well-being within the planetary boundaries; a g

Ämnet nationalekonomi omfattar många områden.Här ska jag lägga fokus på ett av dessa, nämligen makroekonomi. Makroekonomen studerar och förklarar fenomen som konjunktursvängningar, tillväxt, arbetslöshet, ränta, växelkurser, löner och inflation. Makroteorin ligger till grund för förslag om hur samhällsekonomin kan styras genom stabiliseringspolitik. Denna består traditionellt av två grenar: finansÄmnet nationalekonomi omfattar många områden.Här ska jag lägga fokus på ett av dessa, nämligen makroekonomi. Makroekonomen studerar och förklarar fenomen som konjunktursvängningar, tillväxt, arbetslöshet, ränta, växelkurser, löner och inflation. Makroteorin ligger till grund för förslag om hur samhällsekonomin kan styras genom stabiliseringspolitik. Denna består traditionellt av två grenar: finans