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Background: Halomonas boliviensis is a halophilic microorganism that accumulates poly(3-hydroxybutyrate) (PHB) using different carbons sources when nitrogen is depleted from the culture medium. This work presents an improved production of PHB using an air-lift reactor (ALR) that was fed with a concentrated solution of a carbon source, and was supplemented with an adequate airflow rate. Results: Si
Shifting the concept of municipal wastewater treatment to recover resources is one of the key factors contributing to a sustainable society. A novel concept based on research is proposed to recover four main bio-based products from municipal wastewater while reaching the necessary regulatory standards. The main resource recovery units of the proposed system include upflow anaerobic sludge blanket
Background: Lignocellulosic waste is a desirable biomass for use in second generation biorefineries. Up to 40% of its sugar content consist of pentoses, which organisms either take up sequentially after glucose depletion, or not at all. A previously described Escherichia coli strain, PPA652ara, capable of simultaneous consumption of glucose, xylose and arabinose was in the present work utilized fo
This study investigated the efficiency of the treatment processes of wastewater treatment plants (WWTPs) to remove severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and pepper mild mottle virus (PMMoV) from the wastewater and sewage sludge, as well as the influence of the mode of operation on the quality of the treated wastewater. SARS-CoV-2 and PMMoV were detected and quantified at dif
Increased awareness of environmental sustainability with associated strict environmental regulations has incentivized the pursuit of novel materials to replace conventional petroleum-derived plastics. Polyhydroxyalkanoates (PHAs) are appealing intracellular biopolymers and have drawn significant attention as a viable alternative to petrochemical based plastics not only due to their comparable phys
Biotechnologically produced (R)-3-hydroxybutyrate is an interesting pre-cursor for antibiotics, vitamins, and other molecules benefitting from enantioselective production. An often-employed pathway for (R)-3-hydroxybutyrate production in recombinant E. coli consists of three-steps: (1) condensation of two acetyl-CoA molecules to acetoacetyl-CoA, (2) reduction of acetoa
Accumulation of acetate is a limiting factor in recombinant production of (R)-3-hydroxybutyrate (3HB) by Escherichia coli in high-cell-density processes. To alleviate this limitation, this study investigated two approaches: (i) deletion of phosphotransacetylase (pta), pyruvate oxidase (poxB), and/or the isocitrate lyase regulator (iclR), known to decrease acetate formation, on bioreactor cultivati
Wastewater monitoring is highly efficient in SARS-CoV-2 surveillance for tracking virus spread through travel, surpassing traditional airport passenger testing. This study explored the links between SARS-CoV-2 contents and variants from aircraft to city, assessing the impact of detected variants from international travellers versus the local population. A total of 969 variants using next-generatio
Since the rise of radical geography in the early 1970s, political economy has been crucial in examining the spatial dynamics of capital in economic geography. Marxist and non-Marxist (particularly institutionalist) economic geographers use political economy to challenge non-political economy approaches that ignore the relationship between microeconomic processes and their spatial manifestations an
Metabolic dysfunction-associated steatotic liver disease (MASLD) is often asymptomatic early on but can progress to irreversible conditions like cirrhosis. Due to limited access to human liver biopsies, systematic and integrative molecular resources remain scarce. In this study, we performed transcriptomic analyses on liver and metabolomic analyses on liver and plasma samples from morbidly obese i
BACKGROUND: The EAT-Lancet planetary health diet emphasises plant-based foods while including moderate amounts of animal-sourced foods. Simulation-based modelling studies suggest that the diet can provide adequate micronutrients, but concerns remain about potential deficiencies as studies have used different methodologies and few have assessed nutrient intakes using both dietary and biomarker data
In this evaluation of the international legal standing of the right to reparation and its practical implementation at the national level, Christine Evans outlines State responsibility and examines the jurisprudence of the International Court of Justice, the Articles on State Responsibility of the International Law Commission and the convergence of norms in different branches of international law,
Takotsubo syndrome (TTS) is an acute, reversible form of left ventricular dysfunction, typically triggered by emotional or physical stress. The hallmark feature is reversible regional wall motion abnormality extending beyond a single coronary distribution, most commonly presenting with an apical ballooning pattern. The pathophysiology is multifactorial, encompassing neurohormonal dysregulation, ca
The function of the brain is defined by the interactions between its neurons. But these neurons exist in tremendous numbers, are continuously active, and densely interconnected. Thereby, they form one of the most complex dynamical systems known and there is a lack of approaches to characterize the functional properties of such biological neuronal networks without resorting to dimensionality reduct
Groundwater in most parts of the world is an important source of agricultural water supply for food production. Soil salinization, however, threatens groundwater quality and may cause irreparable damage to agricultural land. A key to tackling this threat is to analyze the link between agricultural crop water requirements and groundwater quality. This study aimed to investigate salinity effects on
This chapter reviews methanol's properties as a fuel for marine use in general terms and then specifically for combustion engines and fuel cells. It discusses the different engine technologies being either investigated or demonstrated that allow methanol to be used in marine engines. Their potential in terms of power density, emissions, and efficiency, as well as their maturity are reviewed. The e
Introduction: Child abuse is a global public health problem. It poses a threat to both physical and psychosocial well-being, resulting in serious consequences for the child, the family, and society. Radiographers are in a unique position to identify children at risk. In clinical practice, they may observe unusual interactions between children and their caregivers during radiographic procedures or
Many plant invasions establish from only a few individuals and exhibit clonal spread, providing an opportunity to examine the genomic mechanisms that underlie the success of particularly aggressive individuals. One of the world’s most invasive species, the Japanese knotweed complex, is well suited for such investigations. Simultaneously developing ecological and genomics work in the native and int
The process that generates the different types of highly differentiated cells in the immune system from hematopoietic stem cells involves not only the acquisition of lineage-specific features, but also the permanent repression of alternative developmental programs and the preservation of lineage identity. The well-charted developmental trajectory of early B-cell development, in combination with ab
In this study we investigate day-care centres as a form of community-based support designed to facilitate ageing in place for individuals with dementia, with the goal of delaying or preventing their transition to institutional care. Day-care centres provide structured activities designed to alleviate loneliness and isolation, affirm personal identity, and offer respite for family caregivers. In Sw
