What is CRISPR
CRISPR is a sequence specific gene-editing tool, utilizing a system found in bacteria to introduce double-stranded breaks in the DNA of eukaryotic cells using the endonuclease Cas9.After the cut the break is repaired through non-homologous end-joining (NHEJ) resulting in inserts or deletions (indels) causing gene disruption. If a donor template is present/introduced this can be inserted into the
https://www.stemcellcenter.lu.se/core-facilities/lund-stem-cell-centre-crispr-facility/what-crispr - 2026-04-27
