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A new type of fibre-based anion-exchange material for plasmid purification was developed. The basic material consisted of non-porous silica fibres with a mean diameter of 1.5 mu m and a surface area of 2.4 m(2) g(-1). The fibre surface was provided with several types of ligands, either by adsorption of polymers (chitosan or poly(ethyleneinune)) or by polymerization of amine-containing acrylic mono
OBJECTIVE: The objective of this study was to evaluate whether a regular formula for premature infants supplemented with nucleotides has any influence on plasma lipids and erythrocyte membrane fatty acids. METHODS: Preterm infants fed either human milk supplemented with human milk protein (HM, n = 14), nucleotide-supplemented preterm formula (NF, n = 13), or a regular preterm formula (F, n = 13) w
This study characterizes the high-fat diet-fed mouse as a model for impaired glucose tolerance (IGT) and type 2 diabetes. Female C57BL/6J mice were fed a high-fat diet (58% energy by fat) or a normal diet (11% fat). Body weight was higher in mice fed the high-fat diet already after the first week, due to higher dietary intake in combination with lower metabolic efficiency. Circulating glucose incr
Image registration is the process of geometrically aligning two or more images. In this paper we describe a method for registering pairs of images based on thin-plate spline mappings. The proposed algorithm minimizes the difference in gray-level intensity over bijective deformations. By using quadratic sufficient constraints for bijectivity and a least squares formulation this optimization problem
We compared the penetration depth into cancellous bone when pressurizing cement at predetermined pressure levels, and at different times after cement mixing, in 21 arthrotic femoral heads during total hip replacement. To determine the influence of circulating blood on cement penetration, cement was injected into holes drilled into the femoral head before and after osteotomy of the femoral neck. Th
Three building components exposed to natural climate were studied: a dynamic insulation in the ceiling of a house, an outer wall and one window in the same wall. The term dynamic insulation implies that part of the inlet or exhaust air passes through the insulation of a house. A house with dynamic insulation was continuously measured for approximately a year and a half. The performance of the dyna
The effects of a stretch-release cycle (25 of the resting muscle fibre length, Lo) on both tension and [Ca2+i in small, unstimulated, intact muscle fibre bundles isolated from adult and neonatal rats were investigated at 20 C. The results show that the effects of the length change depended on the age of the rats. Thus, the length change produced three effects in the neonatal rat muscle fibre bundl
Redox dependent protein phosphorylation in chloroplast thylakoids regulates distribution of excitation energy between the two photosystems of photosynthesis, PS I and PS II. Several thylakoid phosphoproteins are known to be phosphorylated on N-terminal threonine residues exposed to the chloroplast stroma. Phosphorylation of light harvesting complex II (LHC II) on Thr-6 is thought to account for re
The first part of the investigation is related to the heat transfer and fluid flow problem inside ribbed ducts with a square cross section. The typical applications of this problem are in the cooling of gas turbine blades and guiding vanes. The second part is related to application of intercoolers in gas turbine systems. The intercooling is performed via intercoolers that usually are water-air hea
The cerebrovascular lesions of severe chronic hypertension were studied by light microscopy in perfusion-fixed, subserially sectioned brains from stroke-prone spontaneously hypertensive rats (SHRSP). The leakage and spread of plasma proteins were visualized by immunohistochemical detection of extravasated fibrinogen and by using an exogenous marker (Evans blue injected i.v.) for blood-brain barrie
