Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus
Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which i