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The emerging field of proteomics has created a need for new high-throughput methodologies for the analysis of gene products. An attractive approach is to develop systems that allow for clonal selection of interacting protein pairs from large molecular libraries. In this study, we have characterized a novel approach for identification and selection of protein-protein interactions, denoted SPIRE (se
Escherichia coli is a microorganism routinely used in the production of heterologous proteins. The overexpression of a xylanase (Xyn10A Delta NC), which originated from the thermophile Rhodo-thermus marinus cloned under the control of the strong T7/lac promoter in a defined medium (mAT) using a substrate-limited feed strategy, was however shown to impose a significant metabolic burden on host cell
Abstract in UndeterminedThe nuisance alga Gonyostomum semen (Raphidophyceae) is known to form dense blooms in many freshwater bodies, and has increased its distribution and abundance. However, information on the mechanisms behind bloom formation and maintenance is scarce. Field observations indicate that G. semen may be favored in humic lakes, In the present study, we performed controlled laborato
Multireference complete active space self-consistent-field (CASSCF) and multireference CASSF second-order perturbation theory (MSCASPT2) calculations were performed on the ground state and a number of low-lying excited singlet and triplet states of chlorobenzene. The dual phosphorescence observed experimentally is clearly explained by the MSCASPT2 potential-energy curves. Experimental findings reg
The efficiency of different preparations of lipases was evaluated in organic solvents. Lipases from Humicola lanuginosa, Candida rugosa, Rhizomucor miehei and Pseudomonas cepacia were adsorbed onto the surfactant sorbitan monostearate (Span 60) and the specific activities were compared in hexane to crude powder (used straight from the bottle) and lipase freeze-dried from buffer solution. Lipases a
Genetic engineering has been used for the fusion of peptides, with different length and composition, on a protein to study the effect on partitioning in aqueous two-phase systems containing thermoseparating polymers. Peptides containing 2-6 tryptophan residues or tryptophan plus 1-3 lysine or aspartate residues, were fused near the C-terminus of the recombinant protein ZZT0, where Z is a synthetic
Pre-treatment serum levels of sCD163 were measured in a cohort of 236 suspected tuberculosis (TB) cases from Guinea-Bissau, with a median follow-up period of 3.3 years (range 0-6.4 years). In 113 cases, the diagnosis of TB was verified by positive sputum microscopy and/or culture. Among the verified TB cases, a decreased survival rate was found in 27 patients with sCD163 levels above the upper ref
Coherent structures and mixing in the flow field of a jet in crossflow have been studied using computational (large eddy simulation) and experimental (particle image velocimetry and laser-induced fluorescence) techniques. The mean scalar fields and turbulence statistics as determined by both are compared for circular, elliptic, and square nozzles. For the latter configurations, effects of orientat
Objective: The course and the long-term outcome of 'refractory' secretory otitis media (SOM), defined as continuous SOM for more than 6 years, were studied in 52 young patients. They had during childhood been treated for bilateral SOM on average for 12 years (range 6 to 26 years). The mean interval between resolution of SOM and examination was 7 years. Methods: At follow up the patients' medical r
This study explores the perceptions of graphic designers regarding the impact of generative artificial intelligence (GenAI) on their profession. It aims to highlight practical examples of how GenAI is integrated into the work of graphic designers. To achieve this, qualitative data was collected through semi-structured interviews with six professional graphic designers. The findings reveal that the
The present study investigates the perceptions and concerns of Lund University students as it pertains to their future employment amidst the rapid development of Artificial Intelligence (AI), which is speculated to have a progressive integration in the labor market. Recent studies project both partial and total task substitution in the coming years, which will have a displacement effect on employm
In this thesis, the effective Hamiltonian formalism is studied and applied to two-photon Rabi oscillations in hydrogen. The process occurs when two photons are absorbed or emitted simultaneously. There exist various approaches to model two-photon transitions. In this thesis, we single out essential quantum states using the projection operator technique and solve for the exact dynamics in this ess
Recent social developments have led to an increased interest in borders. Today, borders are understood as highly dynamic phenomena, that are subject to political-territorial and socio-symbolic interaction. The emergence of new interdisciplinary border studies, as well as advances in technological tools, provide an opportunity for archaeology to examine the cultural phenomenon of boundary marking s
The aim of this thesis is to determine what drives the return in cross-border buy-and-build (B&B) strategies, and if the drivers and the return generated in cross-border B&B strategies differ from those in domestic strategies. Drawing upon prior research of B&B strategies and cross-border M&A, the thesis provides an overview of the current studies on value drivers in private equity
Denna studie undersöker markvattenkoncentrationerna av tre metaller (Cu, Pb, Zn) i jord som behandlats med biokol. Fältförsök har utförts av Statens geotekniska institut sedan 2019 eftersom deras projekt BALANCE undersöker den långsiktiga effekten av biokol i PAH- och metallförorenade jordar. Det långsiktiga målet med studierna är att undersöka om biokol är en effektiv saneringsmetod för förorenadThis study investigates concentrations of three metals (Cu, Pb, Zn) in the soil water of biochar remediated soil. Field trials have been conducted by the Swedish Geotechnical Institution since 2019 as their project BALANCE is exploring the long-term effect of biochar in PAH- and metal contaminated soils. The long-term aim of the studies is to investigate whether biochar amendment is an affective r
In this bachelor thesis, I examine how professional translators handle the Danish discourse particles da, nok, and sgu in translations of Danish fiction texts into Swedish. Of particular interest is how the omission of these discourse particles affects the stylistic character of the text and what implications this has for the reader's experience of the target text. My corpus consists of four D
Climate transition risk pricing in capital markets has emerged as a critical area of research. Using a comprehensive dataset of syndicated loans spanning the years 2010 to 2020, combined with greenhouse gas emission and financial data for borrowers and lenders from three different sources, we conduct an extensive cross-sectional regression analysis to assess how greenhouse gas emissions are priced
Diabetes is a chronic disease that affects lots of people all around the world. It greatly impacts the health of those with it and places many demands on healthcare services. Early detection of diabetes can significantly improve treatment outcomes, lower the chances of future health issues, and help patients to have a better healthier life. In this study, I evaluated the reproducibility of the per
