Removal of a gene in E. coli bacteria Although bacteria are commonly associated with something negative and disgusting it is not the whole truth. It is true that some bacteria can have negative consequences, but as with most things it is unfair to generalize. Bacteria are actually is fascinating organisms. There are many uses for bacteria as well, such as production of protein and other genetic w

ompT - a challenging gene to disrupt in Escherichia coli Our goal in all its simplicity was to delete a gene in two strains of E.coli. ompT is a gene encoding a protease, which is a protein that break down other proteins. We wanted to disrupt ompT and see what effect it could have on recombinant protein production. A method for achieving this is homologous recombination via a system called λ Red

Analys av vattenkvaliteten i floden Rocha belägen i Cochabamba, Bolivia Att det bruna vattnet i Rocha floden som rinner genom Cochabamba-dalen är förorenat råder det ingen tvekan om. Vattenbristen, befolkningsökningen och utsläpp av orenat avloppsvatten ökar föroreningen av den viktiga vattenresursen vilket begränsar användningsområdena och sätter befolkningens och omgivningens hälsa på spel. In this study, a physico-chemical analysis of the Rocha River was performed during the beginning of the rainy season 2018 in order to examine the current state of the water quality in the river at six different locations. The river, located in the Cochabamba department, Bolivia, was examined from upstream in Sacaba to downstream in Sipe Sipe. An additional aim was to determine social and environme

Syftet med denna uppsats är att undersöka hur mediepubliken uppfattar dagens klimatjournalistik samt vilka positiva och/eller negativa känslor klimatjournalistiken kan skapa. Analysen görs utifrån ett sociologiskt perspektiv. Uppsatsens metod är blandad och innehåller en kvalitativ intervjuundersökning och en kvalitativ innehållsanalys. Uppsatsens intervjuresultat av den kvalitativa intervjuunders

Japan lies on an area frequently hit by typhoons while city functions are clustered along the flat coastal areas, hence Japan has experienced lots of storm surge disasters up to the present. Coastal areas along the Suo-nada Sea also have the vulnerability against storm surge and in the past Typhoon 18 in 1999, Typhoon 16 and 18 in 2004 made remarkable damages in the areas. Furthermore, due to glob

The purpose of the study is to examine the attitudes towards criminal records, presented in Flashback in order to gain greater knowledge and understanding of how the criminal record can affect (re)integration. The material consists of posts from the forum Flashback with content about how the criminal record can affect an individual's opportunities in the society and to integrate. The method pe

This paper evaluates the diversification possibilities in emerging markets for a Swedish investor. Two investors with different objectives, one seeks to minimize her risk and one seeks to maximize her risk-adjusted return, are examined. We investigate how non-normal distribution of returns affects the investments and if the benefits of diversification are decreasing over the time period 2006-2018.

In 2012, the Government made an amendment to the Act of alcohol, which meant an exception to the licensing of alcohol on special housing, including for elderly people. The aim of this study was to gather experiences among nursing staff about when elderly’s alcohol consumption becomes problematic and how they argue to solve a problematic situation. Although how the staff of nursing home for elderly

While career-related motives are not given much attention in studies on party membership, there are strong reasons to believe that such professional factors are important for young party members. This study is the first comprehensive investigation of how career-related motives impact the willingness of Swedish leading young party members to become politicians in the future. A unique survey among t

The aim with this essay was to study how professionals within the social services describe collaboration between the different various actors in regard to people with dual diagnosis. This study relies on four qualitative, semi structured interviews with professionals who work with the dual diagnosis target group. The theoretical frame which this study is based upon consists of Berth Danermarks (20

Convolutional Neural Networks impressed the world in 2012 by reaching state-of-the-art accuracy levels in the ImageNet Large Scale Visual Recognition Challenge. The era of machine learning has arrived and with it countless applications varying from autonomous driving to unstructured robotic manipulation. Computational complexity in the past years has grown exponentially, requiring highly efficient

Den matematiska teorin för populationsgenetik utvecklades av Sewell Wright och Ronald Fisher under 1920-30 talet. Wright-Fishers modell för evolution beskriver hur frekvenser av gener i en generation kan beräknas från frekvenserna i den föregående generationen. Förändringar i frekvenserna, evolution, uppkommer på grund av mutationer och selektion, men även på grund av slumpen, så kallad drift. Så Finding the expected number of generations it takes before every individual in a population (of constant size) only has one type of gene at a particular locus of a chromosome, can be formulated as computing the mean absorption time in a Markov chain. However, the Markov chain approach is inappropriate when the population is large. This is commonly solved by approximating the Markov chain with a di

The modern society is getting more and more digitalized and the concept Internet of Things is growing. The data being collected and digital services require a place to be accessed and sold, thus setting the demand for a new kind of marketplace; an online IoT focused marketplace. The large EU supported open source platform for Smart Solutions, FIWARE, has created such a marketplace, namely the Busi

Fertility tracking phone apps are widely used by women today, in order to both achieve or prevent conception. Due to the health risks of hormone based contraceptions, many women opt for non-invasive methods, such as fertility tracking. Most of the algorithms for tracking fertility use parameters such as the basal body temperature (BBT), cycle length and days of menstruation. The widely used "

Title: Significant factors for drug freedom -the perspective of the professionals(translated title) The purpose of this study was to investigate how social workers in the addiction care experienced what factors might be important when it comes to leaving the abuse and achieving drug freedom. The study follows the qualitative method in which four social workers on a support housing for adult addic

During the recent years there have been an increase in sick leave due to mental health issues all over Europe. In Sweden it started to rise in the 90’s and have not stopped since then. Today mental health disorders are the most common reason for sick-leave in Sweden. This applies especially among people working within human services, including social workers. Social workers have a high risk for st

The goal of this thesis work is to implement a convolutional neural network on an FPGA device with the capability of recognising human sign language. The set of gestures that the neural network can identify has been taken from the Swedish sign language, and it consists of the signs used for representing the letters of the Swedish alphabet (a.k.a. fingerspelling). The motivation driving this projec

Uppsatsen undersöker rättskällevärdet för OECD:s riktlinjer om internprissättning för multinationella företag och skattemyndigheter vid tolkning av de s.k. korrigeringsreglerna i svensk och tysk lagstiftning. Genom internprissättning (transfer pricing) avgörs priserna vid transaktioner av fysiska varor och immateriella värden samt utförande av tjänster internt mellan närstående företag i multinatiThis paper examines the legal value of the OECD Transfer Pricing Guidelines for Multinational Enterprises and Tax Administrations as a source of law for the legal systems of Sweden and Germany with regards to internal law regulations enforcing compliance to the arm´s length principle in transfer pricing. Transfer prices are the prices at which enterprises transfer physical goods and intangible pr

This study uses primary survey data to analyze the impact of international remittances on household expenditure across four different districts in the capital city of Ethiopia, Addis Ababa. The impact was explored by testing whether the share of different expenditure categories varies across recipient and non-recipient households. OLS methods were used to assess the impact of international remitta