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In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DN
A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification
Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze
Deterministic Lateral Displacement (DLD) devices have been used to separate particles based on size [1] and shape [2]. Here we show how DLD devices can also be used to separate particles based on their ability to deform under shear forces. Varying experimental conditions allows us to vary the relative contributions of size, morphology and deformability. The ability to distinguish between cells bas
We present a simple and inexpensive device capable of extracting and concentrating the parasite causing sleeping sickness from blood. The device is aimed at being used in rural resource depraved areas where the disease is endemic; therefore simplicity is of paramount importance. The device is based on deterministic lateral displacement with a single inlet and flow induced by a syringe. Through an
Objectives: COPD is a common and disabling disease that entails high costs for society. The objectives of this study were to measure the societal costs of COPD in Sweden, and to examine the relationship between severity of illness and costs. Methods: The costs of COPD were examined using a well-defined and representative cohort of subjects with mild, moderate, and severe COPD. Regular telephone in
How good is your best chimpanzee impression? Go to the zoo and you probably wouldn’t be surprised to see people copying chimpanzees in order to grab their attention. But our latest research, which recently won the Ig Nobel Prize for Anthropology, suggests you are just as likely to see chimpanzees imitating the human visitors.
Background: It remains uncertain whether the increasing incidence of inflammatory bowel disease (IBD) during the last decades has been accompanied by an alteration in the presentation, course, and prognosis of the disease. To answer this question, 3 consecutive population-based IBD cohorts from Copenhagen, Denmark (1962-2005), were assessed and evaluated. Methods: Phenotype, initial disease course
Background: The Stanford integrated psychosocial assessment for transplantation (SIPAT) is a validated psychosocial evaluation tool in the transplant population. Objective: We evaluated SIPAT in predicting post-left ventricular assist device (LVAD) outcomes, including cumulative re-admissions, driveline infections, pump malfunction, pump thrombosis, gastrointestinal bleeding, major bleeding, strok
We present two new data structures for computing values of an n-variate polynomial P of degree at most d over a finite field of q elements. Assuming that d divides q- 1 , our first data structure relies on (d+ 1) n + 2 tabulated values of P to produce the value of P at any of the qn points using O(nqd2) arithmetic operations in the finite field. Assuming that s divides d and d / s divides q- 1 , o
Background: Sentinel lymph node (SLN) metastasis in patients with thin melanomas (≤1 mm) is uncommon but adverse prognostic factors may indicate an increased risk. We sought to determine how often SLN biopsy (SLNB) was performed in patients with thin melanomas, establish the frequency of SLN metastasis and evaluate the predictive value of ulceration, tumor mitotic rate, and thickness for SLN invol
We hypothesized that positive airway pressure treatment would induce nasal obstruction and decrease nasal cavity due to mucosal swelling. We further hypothesized that subjective and objective nasal obstruction at baseline would negatively affect positive airway pressure adherence. A total of 728 patients with sleep apnea were investigated in the Icelandic Sleep Apnea Cohort at baseline and 2 years
Which factors have driven fiscal revenue in Sub-Saharan Africa in the long run? We address this question by studying quantitatively the long-term relationship between fiscal outcomes, economic expansion and external dependency in four francophone West African countries – Benin, Côte d’Ivoire, Niger and Senegal – between 1893 and 2010. Using panel co-integration modelling we find a long-term relati
In 1997 John Broome presented the Collapsing Argument that was meant to establish that non-conventional comparative relations (e.g., “parity,” “imprecise equality,” “incommensurability”) cannot exist. Broome's argument has faced a lot of scrutiny and a certain type of counterexample has been used to undermine it. Most of the counterexamples focus on the Collapsing Principle which plays a central r
The objective of this paper is to present the model-based optimization results of an anaerobic granular sludge internal circulation reactor. The International Water Association Anaerobic Digestion Model No. 1 extended with phosphorus (P), sulfur (S), and ethanol is used to describe the main biological and physico-chemical processes. The high-rate conditions within the reactor are simulated using a
Hydrogen is an element of fundamental importance for energy but hard to quantify in bulk materials. Neutron radiography was used to map . in situ loss of elemental hydrogen from beech tree wood samples during pyrolysis. The samples consisted of three wood cylinders (finished dowel or cut branch) of approximately 1 cm in length. The samples were pyrolyzed under vacuum in a furnace vessel that was p
Understanding and managing the responses of natural systems to climate change requires knowing whether recent changes in ecosystem structure and function are within the bounds of natural variation or whether the systems are entering new states that are unprecedented. Several recent studies of the fossil diatom record of the last ~ 150 years in Andean lakes have shown changes in diatom species comp
Colorectal cancers associated with Lynch syndrome are characterized by defective mismatch repair, microsatellite instability, high mutation rates, and a highly immunogenic environment. These features define a subset of cancer with a favorable prognosis and high likelihood to respond to treatment with anti-programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) drugs. With the aim to define imm
