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Purified and membrane-bound succinate dehydrogenase (SDH) from bovine heart mitochondria was inhibited by the histidine-modifying reagents ethoxyformic anhydride (EFA) and Rose Bengal in the presence of light. Succinate and competitive inhibitors protected against inhibition, and decreased the number of histidyl residues modified by EFA. The essential residue modified by EFA was not the essential
Succinate dehydrogenase consists of two protein subunits and contains one FAD and three iron-sulfur clusters. The flavin is covalently bound to a histidine in the larger, Fp, subunit. The reduction oxidation midpoint potentials of the clusters designated S-l, S-2, and S-3 in Bacillus subtilis wild-type membrane-bound enzyme were determined as +80, -240, and -25 mV, respectively. Magnetic spin int
Large eddy simulation (LES) using a transported PDF model and OH-PLIF/PIV experiments were carried out to investigate the quarl effects on the structures of swirl stabilized methane/air flames. Two different quarls were investigated, one straight cylindrical quarl and one diverging conical quarl. The experiments show that the flames are significantly different with the two quarls. With the straigh
This paper examines information services in association with charging services, and in particular players that offer information services related to charging service offerings from many different operators. It identifies two main types of business models through studying and comparing 25 European charging information service providers. For one type of players the paper analyses the network charact
In this study, we examined the isolation of lignin-carbohydrate complexes (LCCs) from sodium-based spent sulfite liquor, in conjunction with minimization of membrane fouling. We screened 3 polysulfone (PS) membranes with cutoffs of 100, 50, and 25 kDa, respectively. Flux and retention for the 100- and 50-kDa membranes had the same order of magnitude, indicating that these properties were determine
Lactose, glucose and galactose contents in milk and fermented milk products and their lactose-free alternatives, were determined. Storage of products up to the best-before dates had no effect on carbohydrate composition. Total galactose content in yoghurt, i.e., free galactose plus the galactose moiety of lactose, was 2.3–2.4 g 100 g−1 (94–95% of that in milk), whereas that in Swedish soured milk
Steam flow is the main form for energy transfer in power plants, process industries, etc. The flow rate of the steam relates to the energy transfer amount directly. Pressure reducing valves are used for flow rate control of the compressible superheated steam in these piping systems. In this paper, a pressure reducing valve with a novel valve core is proposed. In order to analyze the internal flow
Pilot-control globe valve (PCGV) can use the pressure drop caused by fluid flowing through the orifice located at valve core bottom to open or close the main valve using a small pilot valve. In this paper, computational fluid dynamics (CFD) method is adopted to analyze the pressure drop before and after valve core of PCGV and minor loss of orifice under different structural parameters and inlet ve
Radiocarbon dating is the most commonly used chronological tool in archaeological and environmental sciences dealing with the past 50,000 years, making the radiocarbon calibration curve one of the most important records in paleosciences. For the past 12,560 years, the radiocarbon calibration curve is constrained by high quality tree-ring data. Prior to this, however, its uncertainties increase rap
The challenge of multi-use forestry is to fulfil a range of economic, ecologic and social goals in a sustainable way, accounting for synergies and trade-offs among the ecosystem services provided. Climate changes add to the complexity via effects on forest ecosystem processes, such as primary production and respiration, and also by adding a new goal on the agenda: the role of forests in climate mi
Data that defines IGHV (immunoglobulin heavy chain variable) germline gene inference using sequences of IgM-encoding transcriptomes obtained by Illumina MiSeq sequencing technology are described. Such inference is used to establish personalized germline gene sets for in-depth antibody repertoire studies and to detect new antibody germline genes from widely available immunoglobulin-encoding transcr
Dormant hematopoietic stem cells (dHSCs) display superior serial reconstitution capacity compared to active HSCs, although their role in normal hematopoiesis has not been thoroughly investigated. Recently in Cell, Cabezas-Wallscheid et al. (2017) demonstrate involvement of retinoic acid signaling in murine dHSCs for preservation of the HSC pool.
How are threat images framed and constructed by the so-called warlord democrats (WDs)? Societies that have suffered from large-scale civil wars are commonly permeated by inter-group fear and hate. In these contexts, former military or political leaders of armed groups sometimes become involved in post-war politics. These WDs can act as reconciliation spoilers by making securitising moves, i.e. the
Organisms across the tree of life form symbiotic partnerships with microbes for metabolism, protection and resources. While some hosts evolve extreme dependence on their symbionts, others maintain facultative associations. Explaining this variation is fundamental to understanding when symbiosis can lead to new higher-level individuals, such as during the evolution of the eukaryotic cell. Here we p
In this article, we illustrate the creation of the education policy paradigm that constitutes the framework of vocational education and training (VET) programmes, and analyse local school representatives’ perception of VET in upper secondary schools in Sweden. The education policy paradigm, established through three periods of reform during the twentieth century, undervalues VET as being less wort
In the present work, the spectral properties of gaseous ozone (O3) have been investigated aiming to perform quantitative concentration imaging of ozone by using a single laser pulse at 248 nm from a KrF excimer laser. The O3 molecule is first photodissociated by the laser pulse into two fragments, O and O2. Then the same laser pulse electronically excites the O2 fragment, which is vibrationally ho
Idiopathic Parkinson’s disease (PD) is a complex disease caused by multiple, mainly unknown, genetic and environmental factors. The Ventral root avulsion 1 (Vra1) locus on rat chromosome 8 includes the Glutathione S-transferase alpha 4 (Gsta4) gene and has been identified in crosses between Dark Agouti (DA) and Piebald Virol Glaxo (PVG) rat strains as being associated to neurodegeneration after ne
Here, we introduce a novel scFv antibody, G2-D11, specific for two adjacent Tn-antigens (GalNAc- Ser/Thr) binding equally to three dimeric forms of the epitope, Ser-Thr, Thr-Thr and Thr-Ser. Compared to other anti-Tn reagents, the binding of G2-D11 is minimally influenced by the peptide structure, which indicates a high degree of carbohydrate epitope dominance and a low influence from the protein
Molecules often fragment after photoionization in the gas phase. Usually, this process can only be investigated spectroscopically as long as there exists electron correlation between the photofragments. Important parameters, like their kinetic energy after separation, cannot be investigated. We are reporting on a femtosecond time-resolved Auger electron spectroscopy study concerning the photofragm
