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The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per μg DNA. Seven out of 1
The field of microbial ecology has been developing rapidly, largely owing to the introduction of new molecular methods for identifying individuals, species and populations of microorganisms in nature. In contrast to traditional methods, the new ones do not require the isolation and culture of organisms from the environment. With many of the new methods, genotypes and species of microorganisms can
The uptake and utilization of CH4 at high concentrations (5-18% vol.) and different soil moistures were followed for samples from a landfill cover soil with a high organic matter content. Measurements of the utilization of CH4 and O2, and production of CO2 indicated that the activity of methanotrophic organisms accounted for most of the O2 respiration. At water saturation, CH4 oxidation rates decr
The small subunit (SSU) ribosomal DNA (18S rDNA) from 15 species of nematode-trapping fungi and closely related non-parasitic species were sequenced. Phylogenetic analysis indicated that species within the genera of Arthrobotrys, Dactylaria, Dactylella, Monacrosporium and Duddingtonia formed a monophyletic and isolated clade among an unresolved cluster of apothecial ascomycetes. The phylogenetic p
Landfills account for ca. 10% of the annual global burden of atmospheric methane. Part of the efflux is mitigated by means of biological methane oxidation in the landfill covers. In this study, two types of landfill cover soils (mineral soil and sewage sludge) were compared with respect to methane emissions as well as potential methane oxidation capacity and the PLFA (phospholipid fatty acid) cont
Microbial community-based measurements to detect effects of heavy metals in soils rely on the premise that metal toxicity exerts a selection pressure, which induces a change in the composition of the community. The phospholipid fatty acid (PLFA) composition gives an integrated picture of all living organisms in the soil, and changes in the PLFA pattern of the soil indicate an altered species compo
This article provides evidence for the presence of galectins, previously known as animal lectins, in a fungal species. The important implication of this study is that the galectin gene family could be much older than previously believed. Furthermore, the wide occurrence of galectins increases the possibility of understanding general functions of lectins.
It was recently shown that the nematode-infecting fungus Arthrobotrys oligospora contains a saline-soluble lectin (designated AOL) that is a member of a novel family of fungal lectins sharing similar primary sequences and binding specificities. During saprophytic growth in liquid cultures, levels of AOL and AOL mRNA were found to vary depending on the growth phase of the mycelium and the carbon/ni
It has been proposed that the interactions between several parasite and pathogenic fungi and their hosts are mediated by soluble lectins present in the fungus. We have cloned and analyzed a gene encoding such a lectin (AOL) from the nematophagous fungus Arthrobotrys oligospora (deuteromycete). The deduced primary structure of the AOL gene displayed an extensive similarity (identity 46.3%) to that
Radiolabelled Hg(NO(NO3)2 was added to microcosms containing water and sediment from a lake contaminated with cellulose fibres and mercury. We found that anoxic incubation resulted in methyl 203Hg concentrations in the water at least an order of magnitude higher than the levels in oxygenated incubation. This was explained by the fact that both the production and the water solubility of methyl merc
Several fungi can express high levels of saline-soluble and low- molecular-mass lectins that bind to glycoproteins such as fetuin and different mucins but not bind to any monosaccharides. In this paper, we report the binding specificities of such a lectin (designated AOL) isolated from the nematophagous fungus Arthrobotrys oligospora. The results show that AOL is a multispecific lectin that intera
The nematode trapping fungus Arthrobotrys oligospora produces an extracellular serine protease (designated PII) that immobilizes free-living nematodes in bioassays and hydrolyses proteins of the nematode cuticle. Peptides were isolated from PII and partly sequenced. Three internal peptide sequences were used to design synthetic oligonucleotides, which allowed the subsequent isolation of the gene e
The effects of Zn contamination on the microbial community structure of a forest humus and an arable soil, as estimated by phospholipid fatty acid (PLFA) analysis, were followed during 18 months. The soils were contaminated at 10 different metal concentrations and incubated in plastic jars at 22°C. In both soils effects of heavy metal contamination could be detected after 2 weeks. Qualitatively si
Analysis of phospholipids extracted from various moss and sedge peat types revealed significant differences in the patterns of the phospholipid fatty acids (PLFA) with respect to the major botanical constituents of the peats. Principal component analysis of the PLFA data showed that peats dominated by Carex residues had higher relative amounts of cyclopropane fatty acids and cis vaccenic fatty aci
When grown in liquid cultures allowing the formation of nematode traps, the fungus Arthrobotrys oligospora produced two extracellular proteases hydrolysing the chromogenic substrate Azocoll. The protease activity was separated into two fractions (FI and FII) using anion-exchange chromatography. In bioassays, protease(s) present in FII immobilized the free-living nematode Panagrellus redivivus indi
The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest soil were similar irrespective of which metal was used, while in the arable soil
A major problem in accurately defining bacterial adhesion mechanisms and processes occurring in biofilms on surfaces is the lack of techniques that nondestructively provide on-line information about the microorganisms, their extracellular polymers, and metabolites. The attenuated total reflectance (ATR) technique of Fourier transform infrared spectroscopy (FT-IR) is ideally suited to monitor molec
Several studies have indicated that the capture of nematodes by the nematophagous fungus Arthrobotrys oligospora is mediated by a lectin on the fungal surface. One of the major surface proteins of this fungus showed haemagglutinating activity and was isolated by affinity chromatography using a mucin Sepharose column. Biochemical analysis showed that the protein was a dimeric glycoprotein with a mo
The adhesion mechanisms in three nematophagous fungi are reviewed. In all these fungi the infection and subsequent digestion of nematodes is initiated by the firm adhesion of the fungus to the nematode surface. In Arthrobotrys oligospora the adhesive phase is restricted to special three-dimensional structures. Drechmeria coniospora conidia attach to the nematode cuticle by an adhesive bud while Ca
The nematophagous fungus Arthrobotrys oligospora produced extracellular proteases when grown in a liquid culture, as revealed by measuring the hydrolysis of the chromogenic substrate Azocoll. The extracellular protease activity was inhibited by phenylmethylsulfonyl fluoride (PMSF) and other serine protease inhibitors and partly inhibited by the aspartate protease inhibitor pepstatin and by a cyste
